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2'-O-Methyl-rG (iBu)-Suc-CPG Column

2'-O-Methyl-rG (iBu)-Suc-CPG Column

CPG column for incorporation of a 2'-O-methyl modified ribo-G nucleobase at the 3' end of an oligonucleotide

Key features

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  • CPG has aminopropyl-succinyl linker
  • Standard luer or Supercolumn pipette column types available in different synthesis scales
Option 1: Select a Pore Size
Option 2: Select a Column Type
Option 3: Select a Scale
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Product information

Synthetic oligonucleotides, just like their natural counterparts, are prone to degradation once introduced into a cell. This degradation is due to the presence of exo and endonuclease enzymes, as well as inherent chemical instability (particularly for RNA). Under cellular conditions, this leads to fast in vivo degradation of oligos and a short half-life. (1) To reduce or eliminate this susceptibility, nuclease-resistant modifications can be introduced into oligonucleotides. For antisense or RNAi applications, incorporation of modifications conferring nuclease resistance is essential and such modifications are used routinely. There are a number of ways to introduce nuclease resistance into a synthetic oligonucleotide. Most commonly, the substitution of 2'-OMe bases at some or all positions of an oligo is used as the preferred route to inducing nuclease resistance.(2) Since the nuclease resistance conferred by 2'-OMe lies between that of unmodified nucleosides (no resistance) and phosphorothiolation (highly resistant), extensive/complete 2'-O-methylation is frequently chosen when a high level of nuclease resistance is required. 2'-O-methylation also confers the desirable property of higher binding affinity (that is, higher duplex Tm) to the oligo for its target. For these reasons, 2'-OMe nucleosides are extensively used in siRNA and aptamer applications. 2'-O-Methyloligoribonucleotides are extremely useful reagents for a variety of molecular biology applications. The 2'-OMe RNA-RNA duplex is more thermally stable than the corresponding DNA-RNA one.(3) In addition, 2'-OMe-RNA is chemically more stable than either DNA or RNA and is resistant to degradation by RNA- or DNA-specific nucleases.(4) It is worth noting though that duplexes formed between oligos having 2'-OMe bases at all positions and RNA are incapable of RNase H activity, thus making them ineffective in RNaseH dependent antisense applications,(5) although they can suppress gene expression by blocking the mRNA translation process via steric hindrance.(6) We provide a range of 2'-OMe CPGs with a variety of pore sizes and linkers consistent with our unmodified DNA and RNA CPG products. The protecting group strategies are compatible with the usual DNA and RNA chemistries. Note that the 2'-OMe group in itself is not a 2'-OH protecting group strategy; the 2'-OMe group cannot be cleaved under RNA synthesis and deprotection conditions. Depending on the product, various column types (Standard and ALL-FIT luer, and MerMade, Super and Hybrid pipette columns).


  1. Rate of degradation of {alpha} and {beta}-oligodeoxynucleotides in Xenopus oocytes. Implications for anti-messenger strategies, C. Cazenave, M. Chevrier, T.T. Nguyen and C. Helene, Nucleic Acids Research, 15, 10507- 10521, 1987.
  2. (a) Evaluation of 2'-Modified Oligonucleotides Containing 2'-Deoxy Gaps as Antisense Inhibitors of Gene Expression, B.P. Monia, E.A. Lesnik, C. Gonzalez, W.F. Lima, D. McGee, C.J. Guinosso, A.M. Kawasaki, P.D. Cook and S.M. Frier, J. Biol. Chem., 268, 14514-14522, 1993; (b) Nuclease Resistance and Antisense Activity of Modified Oligonucleotides Targeted to Ha-ras, B.P. Monia, J.F. Johnston, H. Sasmor and L.L. Cummins, J. Biol. Chem., 271, 14533-14540, 1996.
  3. Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides, H. Inoue, Y. Hayase, A. Imura, S. Iwai, K. Miura, and E. Ohtsuka, Nucleic Acids Research, 15, 6131-6148, 1987.
  4. Highly efficient chemical synthesis of 2'-O-methyloligoribonucleotides and tetrabiotinylated derivatives; novel probes that are resistant to degradation by RNA or DNA specific nucleases, B.S. Sproat, A.I. Lamond, B. Beijer, P. Neuner and U. Ryder, Nucleic Acids Research, 17, 3373-3386, 1989.
  5. Sequence-dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H, H. Inoue, Y. Hayase, S. Iwai and E. Ohtsuka, FEBS Lett., 215, 327-330, 1987.
  6. Antisense technologies. Improvement through novel chemical modifications, J. Kurreck, Eur. J. Biochem., 270, 1628-1644, 2003.

Product usage:

  • Cleavage conditions: Use concentrated ammonia for 90 minutes at 25°C or for 30 minutes at 60°C. When using fast deprotecting amidites( CAc; GDMF) 1:1 ammonia:methylamine (AMA) for 25 minutes at 25°C may also be used.
  • Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.Increase time for deprotection if there is an increase in the GiBu content of the oligo.This RNA support is compatible with deprotection chemistries used with 2’ Fluoro; 2' modified phosphoramidites and TBDMS protected reagents.
  • AMA is not recommended for sequences containing uridine bases.Image of cleaved and deprotected structure:
  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 359.23

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: -15 to -30 °C in sealed container

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