dR-DDQ-1 (alpha) CPG
dR-DDQ-1 (alpha) CPG
Key features
Show- Used to add a non-fluorescent quencher to the 3' end of an oligonucleotide.
- Quenches in green region. Alternative to dabcyl and BHQ-1 in certain applications.
- 1000 Å CPG suitable for highly modified oligonucleotides (> 20mers).
Product information
Deep Dark Quencher 1 is a non-fluorescent molecule (so called Dark Quencher) quenching the lower wavelength dyes such as FAM (absorption max. ca. 440 nm). As such, its quenching properties are very similar to that of dabcyl and overlaps, to some degree, with BHQ-1. Thus, for dual-labelled probes, DDQ-1 is not suitable for quenching fluorescent dyes that emit at a higher wavelength, e.g. Cy5®. This modification is available as a 3'-modifier CPG 1000 Å support or internal phosphoramidite. The quencher is attached to the anomeric position of dRibose. This removes the possibility of losing the label during deprotection, a problem often associated with dabcyl due to the 1,2-diol configuration. Additionally, incorporation of DDQ-1 results in preservation of the natural sugar-phosphate backbone meaning there is no adverse effect on the structure of the oligo.
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