3'-Cyanine 5 CPG Column
3'-Cyanine 5 CPG Column
CPG column used to incorporate the cyanine 5 fluorophore at the 3’ end of an oligonucleotide.
Key features
Show- Used to add a red fluorescent dye to the 3' end of an oligonucleotide.
- Maximal emission around 667 nm. Alternative to Quasar 670.
- Quenched by BHQ-2.
- Has DMT group.
- 1000 Å CPG suitable for highly modified oligonucleotides (> 20mers).
Product information
For further details on the use of cyanine-modified products, see our other listed cyanine and Quasar products. The Cyanine 3 and 5 products from the Berry portfolio have the same application as those from LINK (see: LK2412 / LK2413 / LK2520 / LK2521), however they are slightly structurally different in linker construction (aminopropylglycerol) and utilise DMT rather than MMT protection. The cyanine 3 product is also available as an azide (FC8250) for conjugation in e.g. Click Chemistry.
Synthesizer
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Column
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Type/Description
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Notes
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MerMade 6,12
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MerMade, syringe (all scales)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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MerMade 4, 48X, 96E, 192E, 192X
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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ABI 384 / 394
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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Expedite 8909
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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ABI3900
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MerMade
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Pipette type column
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A MerMade column is also known as a Supercolumn
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K&A H4, H8, H8SE, H2, H32, H64
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Luer
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Barrel column with luer fitting at either end
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Also known as ALL-FIT or Standard
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K&A S4CL/S8CL
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Luer
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Barrel column with luer fitting at either end
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Also known as Standard. For this instrument, we recommend the Luer (Standard) column as the ALL-FIT columns have a wider barrel.
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Dr Oligo 48
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MerMade
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Pipette type column
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A MerMade column is also known as a Supercolumn
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Dr Oligo 192XLc, 768XLc just plates
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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OligoMaker X12, 48, 192, X192, X96
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MerMade, Syringe (up to 1.3 mL)
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Pipette type column
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A MerMade column is also known as a Supercolumn
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