Properties:

• Formula: C25H17Cl4NO6
• Molecular Weight: 569.22
• Appearance: yellow solid
• Absorption Maximum (Lambda Max): 519
• Extinction Coefficient at Lambda max: 95000
• Extinction Coefficient at 260 nm: 21800
• Fluorescence Maximum: 537

Spectral properties measured in phosphate-buffered saline (PBS) pH 7.3.

Product usage:

Product Solubility:This product can be dissolved in methanol, DMSO or DMF.Additional Spectroscopic Data:Absorption Maximum: 519 nm (PBS, pH 7.3); 527 nm (MeOH)Fluorescence Emission Maximum: 537 nm (PBS, pH 7.3); 546 nm (MeOH)e coefficient 521 nm = 110,000 cm-1M-1 (MeOH)e coefficient 521nm = 95,000 cm-1M-1 (Water or PBS, pH 7.3)e coefficient 260 nm = 21,800 cm-1M-1 (Water or PBS, pH 7.3)e coefficient 280 nm = 5,600 cm-1M-1 (Water or PBS, pH 7.3)DIPCDI + HOAt Procedure for CAL Fluor Dye Addition to Peptide on Solid PhaseDIPCDI stock solution. 1 mL DIPCDI (di-isopropylcarbodiimide) + 9 mL DMF (HPLC quality).HOAt (1,2,3-Triazolo-[4,5-b]pyridin-3-ol, 1-hydroxyl-7-azabenzotriazole, TCI America T1673), 10 mg solid per 10 mg dye. Procedure. The peptide resin, 20mg, is placed in a syringe reactor (2ml polypro with coarse PE end frit), and the resin swollen in DMF. The dye (10mg) and HOAt (10mg) are placed in a screw capped 1.5 ml vial are treated with 110µL of freshly prepared DIPCDI solution, the vial sealed, and vortexed vigorously for 2 minutes. The dye solution is then diluted with DMF (200µL), mixed and admitted to the reactor. This is covered with foil and shaken overnight. The resin is washed 5x with DMF, treated 1x1 min with 30% piperidine in DMF, washed further with DMF (at least 5x) until no color in washes, washed 5x methanol, and all solvent expelled from syringe. Cleavage reagent (95%TFA/5% H20 made up under argon, 1.5 mL) is admitted to the syringe, which is shaken for 90 minutes. The TFA solution is then expelled into a 20mL glass scintillation vial and evaporated. The residue is dried in vacuo over KOH pellets. The product is analyzed by standard C-18 analytical HPLC in TFA/acetonitrile buffers with a PDA detector.Hazards This procedure, as do all peptide synthesis protocols, involves handling many dangerous, toxic, corrosive, irritant and poisonous chemicals. It should only be performed by a very experienced chemist in a well equipped laboratory fume hood with all possible caution. All waste should be disposed of according to safe laboratory procedures. Particular note should be made of the fact that HOAt is rated as being potentially explosive, it should be handled with care avoiding shock, heat or static electricity. Biosearch Technologies, Inc. assumes no responsibility for the safety of any part of this procedure, which has been supplied strictly for informational purposes only.HATU Procedure for CAL Fluor Dye Addition to Peptide on Solid PhaseDIPEA stock solution 1mL DIPEA (di-isopropylethylamine, glass distilled quality or better) + 9 mL DMF (HPLC quality).HATU [O-(7-Azabenzotriazolo-1-yl)-N,N.N',N'-tetramethyluronium PF6 salt, Aldrich 445460), 10 mg solid per 10 mg dye. Procedure. The peptide resin, 20mg, is placed in a syringe reactor (2 ml polypro with coarse PE end frit), and the resin swollen in DMF. The dye (10 mg) and HATU (10 mg) are placed in a screw capped 1.5 ml vial and treated with 110 µL of freshly prepared DIPEA solution, the vial sealed, and vortexed vigorously for 2 minutes. The dye solution is then diluted with DMF (200 µL), mixed and admitted to the reactor. This is covered with foil and shaken overnight. The resin is washed 5x with DMF, treated 1x1 min with 30% piperidine in DMF, washed further with DMF (at least 5x) until no color in washes, washed 5x methanol, and all solvent expelled from syringe. Cleavage reagent (95%TFA/5% H20 made up under argon, 1.5mL) is admitted to the syringe, which is shaken for 90 minutes. The TFA solution is then expelled into a 20mL glass scintillation vial and evaporated. The residue is dried in vacuo over KOH pellets. The product is analyzed by standard C-18 analytical HPLC in TFA/acetonitrile buffers with a PDA detector.Hazards This procedure involves handling many dangerous, toxic, corrosive and poisonous chemicals. It should only be performed by a very experienced chemist in a well equipped laboratory fume hood with all possible caution. All waste should be disposed of according to safe laboratory procedures. Particular note should be made of the fact that, although HATU is not rated as being potentially explosive, it is potentially just as hazardous as is HOAt, and should be handled with similar care, avoiding shock, heat or static electricity. Biosearch Technologies, Inc. assumes no responsibility for the safety of any part of this procedure, which has been supplied strictly for informational purposes only.

Storage and handling:

• Shipping conditions: Ambient
• Storage conditions: +5 °C, dark and dry