Key featuresShow Hide
- Incorporates a thiol reactive functional group for conjugation internally within an oligonucleotide.
- Avoids need for conversion of e.g. amino functionality to thiol.
- Can be directly conjugated to maleimides and haloacetamides.
Cannot add to favourites
An option must be selected in order to add to favourites
Incorporation of a thiol reactive functional group at specific sites within an oligonucleotide allows for subsequent post-synthesis conjugation of the oligo with a number of different moieties such as fluorescent markers and biotin, depending on the application. Such labels need to be reactive towards the incorporated functional group: for example, thiols will react with iodoacetate and maleimide derivatives to form thioether linkages.
Thiol modification has classically been limited to the 5’ or 3’ end of the oligonucleotide, however it is now possible using this thiol-dT modification that can be incorporated within an oligonucleotide and reacts directly with maleimides and haloacetamides.