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  5. Thiol Modifiers
  6. 3'-Thiol Modifier C6 S-S Glyc CPG

3'-Thiol Modifier C6 S-S Glyc CPG

3'-Thiol Modifier C6 S-S Glyc CPG, 1000 Å, Standard Loading, 1 g

3'-Thiol Modifier C6 S-S Glyc CPG

3'-Thiol Modifier C6 S-S Glyc CPG, 1000 Å, Standard Loading, 1 g

CPG for the incorporation of a thiol function at the 3' end of an oligonucleotide, with a C6 spacer.

Key features

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  • Incorporates a thiol reactive functional group for conjugation.
  • Longer C6 spacer.
  • Glycolate linker for rapid cleavage of the oligonucleotide.
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Item ID BG1-5003-1
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Product information

Incorporation of a thiol reactive functional group at specific sites within an oligonucleotide allows for subsequent post-synthesis conjugation of the oligo with a number of different moieties such as fluorescent markers and biotin, depending on the application. Such labels need to be reactive towards the incorporated functional group: for example, thiols will react with iodoacetate and maleimide derivatives to form thioether linkages. The 3'-thiol-modifier CPGs with C3 and C6 S-S linkers can be used to introduce a 3'-thio functionality, with subsequent cleavage of the disulphide linkage affording the free thiol. This is then available for conjugation.

Properties:

  • Formula: C40H54NO7S2
  • Molecular Weight: 724.99
  • Appearance: white powder

Product usage:

  • Deprotection conditions: Deprotect the oligo in NH4OH for 2 hours at 65°C if using fast deprotecting amidites or 5 hours at 65°C if using standard amidites. This will remove only the base protecting groups.
    • To reduce the disulfide linkage and generate a 3'thiol modified oligo, dry down sample then add 100mM DTT in water. Heat sample for 2 hours, dry the sample down and reconstitute in water. The reduction of the disulfide linkage should be done before the immediate use of the thiol labeled probe to avoid oxidative disulfide formation. To remove the DTT and other side byproducts the oligo can be loaded on to a reverse phase cartridge for desalting. To remove completely DTT include a 5 mL rinse of 5% acetonitrile in 0.1N TEAA before eluting desired oligo in 20% acetonitrile.Image of cleaved and deprotected structure:
  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 328.43

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: -15 to -30 °C

SDS

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