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  6. 3'-Thiol Modifier C6 S-S Glyc CPG Column

3'-Thiol Modifier C6 S-S Glyc CPG Column

3'-Thiol Modifier C6 S-S Glyc CPG Column
3'-Thiol Modifier C6 S-S Glyc CPG Column
3'-Thiol Modifier C6 S-S Glyc CPG Column
3'-Thiol Modifier C6 S-S Glyc CPG Column
3'-Thiol Modifier C6 S-S Glyc CPG Column
3'-Thiol Modifier C6 S-S Glyc CPG Column

3'-Thiol Modifier C6 S-S Glyc CPG Column

CPG column for the incorporation of a thiol function at the 3' end of an oligonucleotide, with a C6 spacer.

Key features

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  • Incorporates a thiol reactive functional group for conjugation.
  • Longer C6 spacer.
  • Glycolate linker for rapid cleavage of the oligonucleotide.
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Option 2: Select a Column Type
Option 3: Select a Scale
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Product information

Incorporation of a thiol reactive functional group at specific sites within an oligonucleotide allows for subsequent post-synthesis conjugation of the oligo with a number of different moieties such as fluorescent markers and biotin, depending on the application. Such labels need to be reactive towards the incorporated functional group: for example, thiols will react with iodoacetate and maleimide derivatives to form thioether linkages. The 3'-thiol-modifier CPGs with C3 and C6 S-S linkers can be used to introduce a 3'-thio functionality, with subsequent cleavage of the disulphide linkage affording the free thiol. This is then available for conjugation. 

Synthesizer
Column
Type/Description
Notes
MerMade 6,12
MerMade, syringe (all scales)
Pipette type column
A MerMade column is also known as a Supercolumn
MerMade 4, 48X, 96E, 192E, 192X
MerMade, Syringe (up to 1.3 mL)
Pipette type column
A MerMade column is also known as a Supercolumn
ABI 384 / 394
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
Expedite 8909
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
ABI3900
MerMade
Pipette type column
A MerMade column is also known as a Supercolumn
K&A H4, H8, H8SE, H2, H32, H64
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
K&A S4CL/S8CL
Luer
  Barrel column with luer fitting at either end 
 Also known as Standard. For this instrument, we recommend the Luer (Standard) column as the ALL-FIT columns have a wider barrel.
Dr Oligo 48
MerMade
Pipette type column
A MerMade column is also known as a Supercolumn
 Dr Oligo 192XLc, 768XLc just plates 
 MerMade, Syringe (up to 1.3 mL) 
Pipette type column
A MerMade column is also known as a Supercolumn
 OligoMaker X12, 48, 192, X192, X96 
MerMade, Syringe (up to 1.3 mL)
Pipette type column
A MerMade column is also known as a Supercolumn

Properties:

  • Formula: C40H54NO7S2
  • Molecular Weight: 724.99
  • Appearance: White Powder

Product usage:

  • Deprotection conditions: Deprotect the oligo in NH4OH for 2 hours at 65°C if using fast deprotecting amidites or 5 hours at 65°C if using standard amidites. This will remove only the base protecting groups.
    • To reduce the disulfide linkage and generate a 3'thiol modified oligo, dry down sample then add 100mM DTT in water. Heat sample for 2 hours, dry the sample down and reconstitute in water. The reduction of the disulfide linkage should be done before the immediate use of the thiol labeled probe to avoid oxidative disulfide formation. To remove the DTT and other side byproducts the oligo can be loaded on to a reverse phase cartridge for desalting. To remove completely DTT include a 5 mL rinse of 5% acetonitrile in 0.1N TEAA before eluting oligo in 20% acetonitrile.Image of cleaved and deprotected structure:
  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 328.43

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: -15 to -30 °C in sealed container

SDS

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