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3'-Glyceryl CPG

3'-Glyceryl CPG

CPG for the incorporation of a glyceryl function at the 3' end of an oligonucleotide for eventual conjugation to amines.
  • Incorporates a glyceryl functionality at 3' end of an oligo
  • Glyceryl functionality is readily oxidized to an aldehyde or carboxylic acid, for conjugation to an amine.
  • 500 Å CPG suitable for high yield applications such as therapeutic oligos (≤ 30mers).
  • 1000 Å CPG suitable for highly modified oligonucleotides (> 20mers).
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Product information

Using glyceryl CPG, the 3'-end of an oligonucleotide is readily oxidized by sodium periodate to form a 3'-phosphoglycaldehyde. The aldehyde may be further oxidized to the corresponding carboxylic acid. Either the aldehyde or the carboxylate may be used for conjugation to amino-functionalised biomolecules. This is an effective strategy for 3' post-synthetic modification with amines, as 3'-functionalised carboxylate and aldehyde solid supports are not commonly available.

Applicable Products

LK2326 3'-Glyceryl SynBase CPG 1000

Physical Data

Item

Mol. Formula

Mol. Wt.

Unit Wt.

LK2236 - - 154.06

Coupling

As with all non-nucleosidic supports, an additional deblock step is required.

Cleavage & Deprotection

This is dependent on the nucleobase protection and other modifications but is typically ammonium hydroxide at 55ºC for 4h, or AMA at 65ºC for 10min.

Storage & Stability

Store dry in a freezer at –10 to –30°C.

References

  1. For oxidation and reduction methods for converting the glycerol group, see: Convenient synthesis of oligonucleotides with a 3’-phosphoglycolate and 3’-phosphoglycaldehyde terminus, H. Urata and M. Akagi, Tetrahedron Lett., 34, 4015-4018 (1993). [Abtract]

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