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  5. Glyceryl Modifiers
  6. 3'-Glyceryl CPG Column

3'-Glyceryl CPG Column

3'-Glyceryl CPG Column
3'-Glyceryl CPG Column
3'-Glyceryl CPG Column
3'-Glyceryl CPG Column

3'-Glyceryl CPG Column

CPG column for the incorporation of a glyceryl function at the 3' end of an oligonucleotide for eventual conjugation to amines.

Key features

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  • Incorporates a glyceryl functionality at 3' end of an oligo
  • Glyceryl functionality is readily oxidized to an aldehyde or carboxylic acid, for conjugation to an amine.
  • 500 Å CPG suitable for high yield applications such as therapeutic oligos (≤ 30mers).
  • 1000 Å CPG suitable for highly modified oligonucleotides (> 20mers).
Option 1: Select a Pore Size
Option 2: Select a Column Type
Option 3: Select a Scale
TBD
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Product information

Using glyceryl CPG, the 3'-end of an oligonucleotide is readily oxidized by sodium periodate to form a 3'-phosphoglycaldehyde. The aldehyde may be further oxidized to the corresponding carboxylic acid. Either the aldehyde or the carboxylate may be used for conjugation to amino-functionalised biomolecules. This is an effective strategy for 3' post-synthetic modification with amines, as 3'-functionalised carboxylate and aldehyde solid supports are not commonly available. 

Synthesizer
Column
Type/Description
Notes
MerMade 6,12
MerMade, syringe (all scales)
Pipette type column
A MerMade column is also known as a Supercolumn
MerMade 4, 48X, 96E, 192E, 192X
MerMade, Syringe (up to 1.3 mL)
Pipette type column
A MerMade column is also known as a Supercolumn
ABI 384 / 394
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
Expedite 8909
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
ABI3900
MerMade
Pipette type column
A MerMade column is also known as a Supercolumn
K&A H4, H8, H8SE, H2, H32, H64
Luer
Barrel column with luer fitting at either end
Also known as ALL-FIT or Standard
K&A S4CL/S8CL
Luer
  Barrel column with luer fitting at either end 
 Also known as Standard. For this instrument, we recommend the Luer (Standard) column as the ALL-FIT columns have a wider barrel.
Dr Oligo 48
MerMade
Pipette type column
A MerMade column is also known as a Supercolumn
 Dr Oligo 192XLc, 768XLc just plates 
 MerMade, Syringe (up to 1.3 mL) 
Pipette type column
A MerMade column is also known as a Supercolumn
 OligoMaker X12, 48, 192, X192, X96 
MerMade, Syringe (up to 1.3 mL)
Pipette type column
A MerMade column is also known as a Supercolumn

Applicable Products

LK2326 3'-Glyceryl SynBase CPG 1000

Physical Data

Item

Mol. Formula

Mol. Wt.

Unit Wt.
LK2236 - - 154.06

Coupling

As with all non-nucleosidic supports, an additional deblock step is required.

Cleavage & Deprotection

This is dependent on the nucleobase protection and other modifications but is typically ammonium hydroxide at 55ºC for 4h, or AMA at 65ºC for 10min.

Storage & Stability

Store dry in a freezer at –10 to –30°C.

References

  1. For oxidation and reduction methods for converting the glycerol group, see: Convenient synthesis of oligonucleotides with a 3’-phosphoglycolate and 3’-phosphoglycaldehyde terminus, H. Urata and M. Akagi, Tetrahedron Lett., 34, 4015-4018 (1993). [Abtract]

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