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5'-Fmoc-Amino C6 Modifier (Fmoc-6-Aminohexyl Amidite)

5'-Fmoc-Amino C6 Modifier (Fmoc-6-Aminohexyl Amidite)

Phosphoramidite for the incorporation of an amino function at the 5' end of an oligonucleotide.

Key features

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  • Incorporates primary amine to 5' end of an oligonucleotide, for subsequent conjugation.
  • Fmoc protection useful where an alternative purification strategy to MMT is needed.
  • The shorter C6 linker may be used to attach compounds where proximity to the oligonucleotide causes no problem.
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Product information

Incorporation of a primary amine reactive functional group at specific sites within an oligonucleotide allows for subsequent post-synthesis conjugation of the oligo with a number of different affinity, reporter or protein labels, depending on the application. Such labels need to be reactive towards the incorporated functional group: for example, NHS esters or isothiocyanates will react with primary amines. This approach is often necessary where the desired label or tag is either not available as a phosphoramidite, or is sensitive or unstable to the conditions of oligonucleotide synthesis or deprotection. A common example is the attachment of a rhodamine dye using the TAMRA NHS ester. Functionally-derivatised oligos can also be covalently attached to surfaces such as glass slides or gold microspheres for use in various microarray or nanoelectronic applications. The Fmoc-protected amino-C6 product is useful where incorporation of a primary amine at the 5'-terminus of the oligonucleotide using an ‘amino-linker’ phosphoramidite requires an alternative purifcation or application strategy outwith the normal base labile trifluoroacetate (TFA) and acid-labile monomethoxytrityl (MMT) options.

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