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DNP-TEG CE-Phosphoramidite

DNP-TEG CE-Phosphoramidite

CAS No.:1027512-01-1

Phosphoramidite used to incorporate a dinitrophenyl-modification, either internally or at the terminus of an oligonucleotide.

Key features

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  • Useful in colourimetric analytical detection methods.
  • Tetraethylene glycol spacer affords hydrophilicity.
  • Branched structure allows multiple additions at either 3' or 5' terminus.
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Product information

Colourimetric detection is one of the oldest diagnostic techniques. This is based on the interaction of an enzyme e.g. HRP interacting with a substrate. This also requires some form of capture of the target with a hapten labelled probe and the duplex captured using an affinity column or matrix loaded with a suitable protein or antibody. Examples of haptens are biotin, DNP and DIG, the most commonly used being biotin in conjunction with streptavidin or avidin. The enzyme labelled oligo then hybridises to another part of the immobilised target and treatment with the substrate produces a distinctive colour.

An analytical test based on detection of 2,4-dinitrophenyl (DNP) labelled oligonucleotides with anti-DNP antibodies has been reported.(1) The branched tetraethylene glycol spacer in DNP-TEG phosphoramidite allows multiple additions to the 3' or 5' terminus of an oligonucleotide.


  1. The synthesis of oligonucleotides that contain 2,4-dinitrophenyl reporter groups, D.W. Will, C.E. Pritchard, and T. Brown, Carbohydrate Research, 216, 315-322, 1991.

Applicable Products

LK2549 DNP-TEG-CE Phosphoramidite

Physical & Dilution Data

Dilution volumes (ml) are shown for 0.1M solutions in dry acetonitrile (4050). Adjust accordingly for other concentrations. For µmol pack sizes, products are diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.


Mol. Formula

Mol. Wt.

Unit Wt.




LK2549 C48H64N5O13P 950.04 509.41 2.63 5.26 10.53

Coupling, Deprotection & Purification

A 12-15min coupling time is recommended. Most deprotection conditions are applicable. For optimal yield, oligos are prepared DMT ON, with removal of the DMT group after cleavage and deprotection. Otherwise, the 1,2-diol configuration enables cleavage of the DNP-TEG during deprotection. A pre-treatment of the column with 10% diethylamine in acetonitrile for 2min at room temperature (2x) to remove cyanoethyl protecting groups, then a rinse with acetonitrile, can minimise the unwanted DNP-TEG cleavage.

Storage & Stability

Store in a freezer at –10 to –30°C. Stable in solution for 2-3 days.

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