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Desthiobiotin-TEG CPG

Desthiobiotin-TEG CPG

CPG that allows affinity capture via the biotin-streptavidin interaction with the ability to recover the material.
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Product information

Desthiobiotin allows capture by streptavidin and can be displaced simply by adding biotin.(1) This is available as the ester (BT1070)(2), phosphoramidite (BA0210), CPG (BA0211) and azide (BT1075) versions.
Regarding association constants and kinetics, see: (a) Busse, S.; Scheumann, V.; Menges, B.; Mittler, S. Biosensors and Bioelectronics, 2002, 17, 704-710. (b) Yoon, H.C.; Hong, M.-Y.; Kim, H.-S. Langmuir, 2001, 17, 1234-1239.
For desthiobiotin itself see BT1060.

Ref:

  1. Hirsch, J.D.; Eslamizar, L.; Filanoski, B.J.; Malekzadeh, N.; Haughland, R.P.; Beechem, J. M.; Haughland, R.P. Anal. Biochem. 2002, 308, 343-357.
  2. (a) Hofmann, K.; Titus, G.; Montibeller, J.A.; Finn, F.M. Biochemistry, 1982, 21, 978-984. (b) Romovacek, H.; Finn, F.M.; Hofmann, K. Biochemistry, 1983, 22, 904-909. (c) Finn, F.M.; Titua, G.; Hofmann, K. Biochemistry, 1984, 23, 2554-2558.

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