Desthiobiotin N-Hydroxysuccinimidyl Ester
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Desthiobiotin N-Hydroxysuccinimidyl Ester
CAS No.:80750-24-9
For affinity capture via the biotin-streotavidin interaction.
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Desthiobiotin allows capture by streptavidin and can be displaced simply by adding biotin.(1) This is available as the ester (BT1070)(2), phosphoramidite (BA0210), CPG (BA0211) and azide (BT1075) versions.
Regarding association constants and kinetics, see: (a) Busse, S.; Scheumann, V.; Menges, B.; Mittler, S. Biosensors and Bioelectronics, 2002, 17, 704-710. (b) Yoon, H.C.; Hong, M.-Y.; Kim, H.-S. Langmuir, 2001, 17, 1234-1239.
For desthiobiotin itself see BT1060.
Ref:
- Hirsch, J.D.; Eslamizar, L.; Filanoski, B.J.; Malekzadeh, N.; Haughland, R.P.; Beechem, J. M.; Haughland, R.P. Anal. Biochem. 2002, 308, 343-357.
- (a) Hofmann, K.; Titus, G.; Montibeller, J.A.; Finn, F.M. Biochemistry, 1982, 21, 978-984. (b) Romovacek, H.; Finn, F.M.; Hofmann, K. Biochemistry, 1983, 22, 904-909. (c) Finn, F.M.; Titua, G.; Hofmann, K. Biochemistry, 1984, 23, 2554-2558.
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