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D-Desthiobiotin

D-Desthiobiotin

CAS No.: 533-48-2

Used in affinity capture via the biotin-streotavidin interaction.
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Desthiobiotin allows capture by streptavidin and can be displaced simply by adding biotin.(1) For phosphoramidite and CPG versions, see BA0210 and BA0211. For the NHS ester of desthiobiotin, see BT1070.

Ref:

  1. (a) General: Hirsch, J.D.; Eslamizar, L.; Filanoski, B.J.; Malekzadeh, N.; Haughland, R.P.; Beechem, J. M.; Haughland, R.P. Anal. Biochem. 2002, 308, 343-357. (b) Key references on Desthiobiotin NHS Ester: (i) Hofmann, K.; Titus, G.; Montibeller, J.A.; Finn, F.M. Biochemistry, 1982, 21, 978-984. (ii) Romovacek, H.; Finn, F.M.; Hofmann, K. Biochemistry, 1983, 22, 904-909. (iii) Finn, F.M.; Titua, G.; Hofmann, K. Biochemistry, 1984, 23, 2554-2558. (c) Regarding association constants and kinetics: (i) Busse, S.; Scheumann, V.; Menges, B.; Mittler, S. Biosensors and Bioelectronics, 2002, 17, 704-710. (ii) Yoon, H.C.; Hong, M.-Y.; Kim, H.-S. Langmuir, 2001, 17, 1234-1239.
Brand Berry
Modification Biotin

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