Desthiobiotin allows capture by streptavidin and can be displaced simply by adding biotin.⁽¹⁾ For phosphoramidite and CPG versions, see BA0210 and BA0211. For the NHS ester of desthiobiotin, see BT1070.

Ref:

1. (a) General: Hirsch, J.D.; Eslamizar, L.; Filanoski, B.J.; Malekzadeh, N.; Haughland, R.P.; Beechem, J. M.; Haughland, R.P. Anal. Biochem. 2002, 308, 343-357. (b) Key references on Desthiobiotin NHS Ester: (i) Hofmann, K.; Titus, G.; Montibeller, J.A.; Finn, F.M. Biochemistry, 1982, 21, 978-984. (ii) Romovacek, H.; Finn, F.M.; Hofmann, K. Biochemistry, 1983, 22, 904-909. (iii) Finn, F.M.; Titua, G.; Hofmann, K. Biochemistry, 1984, 23, 2554-2558. (c) Regarding association constants and kinetics: (i) Busse, S.; Scheumann, V.; Menges, B.; Mittler, S. Biosensors and Bioelectronics, 2002, 17, 704-710. (ii) Yoon, H.C.; Hong, M.-Y.; Kim, H.-S. Langmuir, 2001, 17, 1234-1239.