5'-POM-vinyl phosphonate, 2'-OMe-U CE-Phosphoramidite
5'-POM-vinyl phosphonate, 2'-OMe-U CE-Phosphoramidite
5'-POM-vinyl phosphonate, 2'-OMe-U CE-Phosphoramidite, 250 mg, ABI (8 mL / 20 mm Septum)
Key features
Show- Metabolically stable 5’ vinyl phosphonate mimics 5’ phosphorylation.
- POM protection allows clean and rapid one-step deprotection with addition of 3% (v/v) diethylamine to the standard aqueous ammonia deprotection solution, minimizing side reactions and impurities.
- Available in small pack sizes for research use
- Stringent QC includes testing for synthesis coupling efficiency
Usually shipped in 3-5 days, Packed To Order
Product information
The standard dU nucleobase is chemically ligated with a hydrolytically impervious 5’ vinyl phosphonate (VP), an effective bioisostere of the natural 5′-monophosphate in small interfering RNAs (siRNAs). VPs are shown to enhance RNA-induced silencing complex (RISC) recognition and thus avoid the use of metabolically unstable 5’ phosphates. There is some evidence to suggest enhancement of exonuclease stability. Of particular use where a sequence is incompatible with cellular kinases and thus cannot be 5’ phosphorylated in-vitro. (1)
Two options are available, either OMe or POM protection. 5'-[O,O-bis(pivaloyloxymethyl)] (POM) protection allows clean and rapid one-step deprotection with addition of 3% (v/v) diethylamine to the standard aqueous ammonia deprotection solution, minimizing side reactions and impurities.
Ref:
- Jadhav et al. ChemBioChem 2016, 17, 985 – 989.
Access support
Need some support with placing an order, setting up an account, or finding the right protocol?
Contact us