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SuperPure Plus Purification Column

SuperPure Plus Purification Column

For reverse-phase purification of trityl-protected oligonucleotides followed by on-column detritylation (> 200 nmol scale).
  • High performance hydrophobic polymeric resin.
  • Removes the most common impurities and truncated sequences
  • The method is rapid, efficient and many samples can be purified simultaneously.
  • Suitable for 200 nmol scale
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Product information

SuperPure Plus™ Purification Columns are intended for reverse-phase purification of trityl-protected oligonucleotides followed by on-column detritylation. The method relies on strong binding between the trityl-protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and many samples can be purified simultaneously. SuperPure Plus™ is suitable for purification at the 200 nmol scale. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.

Product usage:

  1. Program DNA synthesizer to leave terminal 5'-DMT group attached;
  2. Cleave and deprotect product with conc. aqueous ammonia (5 hours at 60°C for standard deprotectionprocedure);
  3. Evaporate cleavage solution to dryness;
  4. Re-suspend in 0.5ml 0.1N TEAA or H2O;
  5. Pre-equilibrate column by passing 2 column volumes (CVs) of acetonitrile through the column;
  6. Repeat with 3 CVs 1N TEAA (triethylamine acetate);
  7. Take the oligo solution of step 4 and apply slowly to the column while collecting effluent in a separatemicro-centrifuge tube. Reload effluent and pass once more through column (optional);
  8. Pass 3 CVs of 2.8% ammonia solution slowly through the column;
  9. Wash by slow passage of 2 CVs water;
  10. Pass 2 CVs of 2.5% TFA through the column, wait 5 minutes;
  11. Pass 3 or more CVs of water through column. Check to see pH is neutral.
  12. Slowly pass 2 CVs 20% acetonitrile through the column, collecting effluent containing the desiredpurified product in a labeled microcentrifuge tube.
  13. Speedvac ™ the solution to dryness, analyze and use as desired.

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: Room Temperature

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