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Fluorous Chemical Phosphorylation Reagent II (F-CPR-II CEP) allows a combination of fluorous affinity purification with concomitant 5'-phosphorylation, which is especially attractive when synthesizing longmers that will be used in ligase reactions. Further, if a 5'-phosphate is tolerated in a particular application, F-CPR-II may be used as a common handle for fluorous purification, obviating the need to choose a different fluorous phosphoramidite for each different 5'-terminal nucleotide. F-CPR-II CEP is used in the same way as CPR-II (LK2110)(1), i.e., a 6 minute coupling with no capping. Use with Fluoro-Pak™ Columns (FP7210 or FP7220) and Loading Buffer (LB7100). The final retro-aldol cleavage to afford the free 5'-phosphorylated oligonucleotide is achieved in the same fashion as found for CPR-II.
- Guzaev, A., et al., Tetrahedron 1995, 51, 9375-9384; US Patent 5,959,090)