Skip to content Skip to navigation menu

Exo-Minus Klenow DNA Polymerase (D355A, E357A)

Product Details

Show Hide

The Exo-Minus Klenow DNA Polymerase (D355A, E357A) Kits include Exo-Minus Klenow DNA Polymerase (D355A, E357A) at 10 U/µL and 10X Reaction Buffer.

The "enzyme only" format contains Exo-Minus Klenow DNA Polymerase (D355A, E357A) at 10 U/µL.

Exo-Minus Klenow DNA Polymerase (D355A, E357A)

Exo-Minus Klenow DNA Polymerase (D355A, E357A) Kit, 10 U/µL. 1,000 U

A Klenow DNA polymerase lacking all 5' to 3' and 3' to 5' exonuclease activity.

Key features

Show Hide
  • A Klenow DNA polymerase lacking all 5' to 3' and 3' to 5' exonuclease activity
  • Synthesise DNA from ssDNA templates without degrading any single-stranded DNA
  • Suitable for a variety of applications including random-primer labelling of DNA,1second-strand cDNA synthesis,2sStrand-displacement amplification3
Option 1: Select a Format
Option 2: Select a Size
Item ID KL11101K
TBD
Add to basket to request a quote

Product information

Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´ to 3´ and 3´ to 5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I also has two mutations (D355A and E357A).

References

  1. Feinberg, A.P. and Vogelstein, B. (1984) Anal. Biochem. 137, 266.
  2. Sanbrook, J. et al. (1989) In: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York. v.2, p. 8.14.
  3. Walter, N.G. (1995) J. Mol. Biol. 254, 856.

Access support

Need some support with placing an order, setting up an account, or finding the right protocol?

Contact us