We offer two bacterial strains designed for BAC library construction or transformation of large constructs:

BAC-Optimised Replicator™ v2.0 Electrocompetent Cells (> 1 × 10¹⁰ cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are components of CopyRight^® Cloning Kits.

E. cloni 10G BAC-Optimised Electrocompetent Cells (> 1 × 10⁷ cfu/µg 150 kb BAC DNA; > 1 × 10¹⁰ cfu/µg pUC DNA). The first competent cells developed specifically for large DNA cloning. Manufactured using a novel procedure that maximises transformation efficiencies with very large inserts, 10G BAC-Optimised Cells are an excellent choice for BAC library construction and large construct transformation.

Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning