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NxSeq SARS-CoV-2 Whole Genome Library Kit and Indexing Plates

NxSeq SARS-CoV-2 Whole Genome Library Kit and Indexing Plates

A fast and efficient, amplicon-based SARS-CoV-2 whole genome library preparation system for Illumina sequencers
  • Identify both known and novel variants: 98% SARS CoV-2 genome coverage
  • Fast, easily automated protocol: 2 hours from cDNA to final library with minimal steps
  • Extensive compatibility across Illumina sequencers: Small amplicons enable 2 x 150 PE sequencing chemistry available on more Illumina platforms (compared to amplicon panels >350 bp in size, e.g. ARTIC)
  • High multiplexing drives down costs: 1440 CDI indexes plus uniform coverage, together maximize multiplexing capabilities and simplify analysis.

Select the quantities you need, then add to basket

Product description Favourite Size Item ID Price Quantity
Product description: NxSeq SARS-CoV-2 Whole Genome Library Kit, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-SCV2-0096
Product description: Indexing Plate 1, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-001
Product description: Indexing Plate 2, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-002
Product description: Indexing Plate 3, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-003
Product description: Indexing Plate 4, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-004
Product description: Indexing Plate 5, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-005
Product description: Indexing Plate 6, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-006
Product description: Indexing Plate 7, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-007
Product description: Indexing Plate 8, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-008
Product description: Indexing Plate 9, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-009
Product description: Indexing Plate 10, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-010
Product description: Indexing Plate 11, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-011
Product description: Indexing Plate 12, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-012
Product description: Indexing Plate 13, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-013
Product description: Indexing Plate 14, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-014
Product description: Indexing Plate 15, 96 reactions Favourite: 96 reactions
Delivered amount: GEN-9754-015

Product information

The NxSeq™ SARS-CoV-2 Whole Genome Library Kit supplies the targeting primers, enzymes and other reagents necessary to build overlapping, targeted amplicon libraries covering the SARS-CoV-2 genome. These libraries enable whole genome viral sequencing on Illumina instruments for the identification and tracking of SARS-CoV-2 variants. We designed the targeting primer pairs against the Wuhan-Hu-1 strain (NC_045512.2) and carefully screened and optimized the primer set to produce high on-target rates and consistent amplicon yields in order to maximize multiplexing, streamline analysis and keep costs low. Each amplicon target region* overlaps with the target regions of adjacent amplicons in order to generate nearly full coverage and effective variant analysis of each SARS-CoV-2 isolate.

Key kit features 

Feature Details
Input Single-stranded or double-stranded cDNA made from positive RNA samples
SARS-CoV-2 genome coverage 98% or 29,313 / 29,903 total nucleotides of viral RNA genome
Amplicon number 341 overlapping amplicons
Amplicon sizes 101 nt average target region* length making it compatible with common 2x150 PE sequencing chemistry
Protocol time 2 hours from cDNA to final library
Components provided** SARS-CoV-2 targeting primers, PCR and library prep components
Index style 15 Indexing plates are available, each with 96 different combinatorial dual indexes
Multiplexing levels Up to 1440 samples when using all 15 different indexing plates
Recommended sequencing depth ~1 million reads per library – may be reduced depending on sample quality

*Target region refers the portion of each amplicon between the 3’ ends of each forward and reverse primer pair. This portion of each amplicon that will provide the useable variant information, and each target region overlaps with an adjacent target region.

** cDNA synthesis reagents and clean-up/size selection beads are not included

For Research Use Only. Not for use in diagnostic procedures. 

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