8-oxo-dG(iBu) CE-Phosphoramidite
8-oxo-dG(iBu) CE-Phosphoramidite
8-oxo-dG(iBu) CE-Phosphoramidite, BULK (g), HDPE Screw-Top
CAS No.:143060-53-1
Key features
Show- Useful for mutagenic studies involving oxidative processes or ionisation radiation.
- Classical isobutyryl protection.
Usually shipped in 3-5 days, Packed To Order
Product information
8-oxo-dG-CE Phosphoramidite allows investigation of the structure and activity of oligonucleotides containing an 8-oxo mutation. This is formed naturally when DNA is subjected to oxidative conditions or ionising radiation. The resulting 8-oxo modification is significant in mutagenesis and ultimately carcinogenesis.
Applicable Products
| LK2072 | 8-oxo-dG-CE Phosphoramidite |
Physical & Dilution Data
Dilution volumes (in ml) are for 0.1M solutions in dry acetonitrile (LK4050). Adjust accordingly for other concentrations. For µmol pack sizes, products should be diluted as 100µmol/ml to achieve 0.1M, regardless of molecular weight.
Item |
Mol. Formula |
Mol. Wt. |
Unit Wt. |
250mg |
500mg |
1g |
| LK2072 | C44H54N7O9P | 855.93 | 345.21 | 2.92 | 5.84 | 11.68 |
Coupling
No changes are required from the standard method recommended by the synthesiser manufacturer. Coupling is as per standard nucleoside amidites.
Deprotection
Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol for 17h at 55°C to avoid oxidative degradation of the 8-oxo-dG site.
Storage & Stability
Store in the fridge, dry. Stability in solution is 24h.
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