Skip to content Skip to navigation menu

RapiDxFire Thermostable Reverse Transcriptase 3 Units/µL

RapiDxFire Thermostable Reverse Transcriptase 3 Units/µL

RapiDxFire Thermostable Reverse Transcriptase 3 Units/µL

Generate short cDNAs (<1 kb) quickly at temperatures > 55°C. 

Select a number of reactions
Item ID 30250-2
TBD
Add to basket to request a quote

Product information

RapiDxFire™ Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10 min at 90⁰C) and maintains its core activity after >3 months storage at 20-25⁰C. This enzyme lacks RNAse H and 3’ 5’ exonuclease activity and efficiently synthesizes short cDNA fragments (≤ 1 Kb). Like other reverse transcriptases, RapiDxFire RT possesses DNA polymerase activity but lacks 5′ 3′ exonuclease activity. Supplied in a glycerol-free, Triton™ X-100-free storage buffer, the enzyme formulation can be further optimized for downstream lyophilization.

Access support

Need some support with placing an order, setting up an account, or finding the right protocol?

Contact us