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  5. Premixed dNTP Solutions

Premixed dNTP Solutions

Includes dATP, dCTP, dGTP and dTTP. Concentrations and sizes are for each dNTP.

Premixed dNTP Solutions

dNTP (All 4 mixed), 25 mM ea., 10 µmole

Ready-to-use pre-mixed dNTP solutions for PCR and other DNA synthesis reactions.

Key features

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  • Sterile, neutral pH solutions of premixed dGTP, dATP, dTTP and dCTP
  • Available at two different concentrations of each dNTP (2.5 mM or 25 mM)
Select a Concentration
Item ID D59104
TBD
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Product information

Sterile, neutral pH, pre-mixed solutions of dATP, dCTP, dGTP, and dTTP are available for use in DNA synthesis reactions including PCR. The pre-mixed dNTPs solutions are available at two concentrations, either 2.5 mM or 25 mM of each dNTP. Each solution is sufficient for at least 1,000 50 µL reactions at concentrations used in typical reactions with most DNA polymerases.

Storage Buffer: dNTP solutions are made with sterile deionised water and adjusted to pH 7.0.

Quality Control: All Epicentre dNTP solutions meet spectrophotometric criteria and are function-tested using our DNA polymerases.

Table 1: Molecular Characteristics of dNTPs

dATP (2´-Deoxyadenosine-5´-Triphosphate)
Formula (free acid): C10H16N5O12P3

Molecular weight: 491
A250/A260 = 0.80
A280/A260 = 0.15

dCTP (2´-Deoxycytidine-5´-Triphosphate)
Formula (free acid): C9H16N3O13P3

Molecular weight: 467
A250/A260 = 0.84
A280/A260 = 0.98

dGTP (2´-Deoxyguanosine-5´-Triphosphate)
Formula (free acid): C10H16N5O13P3

Molecular weight: 507
A250/A260 = 1.15
A280/A260 = 0.66

dTTP (2´-Deoxythymidine-5´-Triphosphate)
Formula (free acid): C10H17N2O14P3

Formula weight: 482
A250/A260 = 0.65
A280/A260 = 0.71

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