Skip to content Skip to navigation menu

Universal Support-CPG (DMT-Ribose-Linker)

Universal Support-CPG (DMT-Ribose-Linker)

Universal CPG solid support for oligonucleotide synthesis, with a ribose linker.
  • Ribose linker.
  • Contains DMT functionality.
  • Requires elevated temperatures and/or elongated time to completely remove linker.
  • Choice of pore sizes.
Option 1: Select a Pore Size
Option 2: Select a Functional Loading
Option 3: Select a Size
Add to basket to request a quote

Product information

There are advantages in using a ‘universal’ support where there is no nucleobase or modification already present (e.g. when using plate synthesizer). In this case, the first base at the 3'-end is determined by the first phosphoramidite addition in the synthesis cycle. When preparing wells in plate synthesizers this eliminates the possibility of the incorrect resin being placed in a well. It also allows automated preparation of the plates to stock ready for synthesis. There is an added benefit in large scale syntheses, where supply chain is simplified as only the one support is required. A universal support can also be applied in situations where a 3'-modification support is not available, using a phosphoramidite modifier as the first addition in the cycle (this will only work with phosphoramidites capable of extending the oligo chain, i.e. not 5'-modifiers and are compatible with the universal support cleavage and deprotection conditions). For these purposes we offer the original universal CPG (1), plus alternative products with hydroquinone (“Q”) and ribose linkers. The Q-supports were developed (2) with fast, mild cleavage in mind, however we have observed mixed results. The linker is stable to capping mixtures, but is slightly labile in oxidiser solution (8% cleavage overnight which is the equivalent of approximately 2000 synthesis cycles on an average program). In all cases for these universal supports, a high temperature or extended time is required to completely remove the universal linker. For this reason it is inadvisable to use these supports with modifications that require mild, ultra mild or room temperature deprotection. Compatibility with RNA is therefore mixed, and we would not recommend use with TBDMS RNA chemistry.


  1. A universal support for oligonucleotide synthesis, S. Scott, P. Hardy, R.C. Sheppard, and M.J. McLean, in Innovation and Perspectives in Solid-Phase Synthesis. Peptides, Proteins, and Nucleic Acids, Biological and Biomedical Applications (R. Epton, ed.), 115-124, 1994. Mayflower Worldwide, Ltd., Birmingham, UK.
  2. Hydroquinone-O,O'-diacetic acid as a more labile replacement for succinate acid linkers in solid-phase oligonucleotide synthesis, R.T. Pon and S.Y. Yu, Tetrahedron Lett., 38, 3327-3330, 1997.


  • Appearance: white powder

Product usage:

  • Cleavage conditions: For cleavage use ammonia/LiCl (15 mg LiCl in 1 mL concentrated aqueous ammonia, ca 0.1N LiCl) or methylamine/ammonia/LiCl for 90 minutes at 25°C. Filter off CPG support and collect supernant.
  • Deprotection conditions: Deprotect supernant in ammonia/LiCl (15 mg LiCl in 1 mL concentrated aqueous ammonia, ca 0.1N LiCl) for 6 hours at 65°C or methylamine/ammonia/LiCl for about 3-4 hours at 65°C.
  • Purification and Analysis: If the trityl has previously been removed from the oligo, the lithium salt is removed by precipitation from cold ethanol/salt or using Sephadex G-25 filtration (NAP). Alternatively, the crude TRITYL-ON oligonucleotide can be desalted and purified using commercially available reverse phase oligo purification cartridges (MicroPure II, BTI MP-1602).
  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 0

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: +2 to +8 °C

Access support

Need some support with placing an order, setting up an account, or finding the right protocol?

Contact us