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Universal-Q CPG Column

Universal-Q CPG Column

Universal CPG solid support column for oligonucleotide synthesis, with a Q linker.
  • Hydroquinone linker.
  • Contains DMT functionality.
  • Can be used in UltraMILD or FAST conditions but requires elevated temperatures and/or elongated time to completely remove linker.
  • Choice of pore sizes and column formats.
Option 1: Select a Pore Size
Option 2: Select a Column Type
Option 3: Select a Scale
TBD
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Product information

There are advantages in using a ‘universal’ support where there is no nucleobase or modification already present (e.g. when using plate synthesizer). In this case, the first base at the 3'-end is determined by the first phosphoramidite addition in the synthesis cycle.

When preparing wells in plate synthesizers this eliminates the possibility of the incorrect resin being placed in a well. It also allows automated preparation of the plates to stock ready for synthesis. There is an added benefit in large scale syntheses, where supply chain is simplified as only the one support is required.

A universal support can also be applied in situations where a 3'-modification support is not available, using a phosphoramidite modifier as the first addition in the cycle (this will only work with phosphoramidites capable of extending the oligo chain, i.e. not 5'-modifiers and are compatible with the universal support cleavage and deprotection conditions).

For these purposes we offer the original universal CPG (1), plus alternative products with hydroquinone (“Q”) and ribose linkers.

The Q-supports were developed (2) with fast, mild cleavage in mind, however we have observed mixed results. The linker is stable to capping mixtures, but is slightly labile in oxidiser solution (8% cleavage overnight which is the equivalent of approximately 2000 synthesis cycles on an average program).

In all cases for these universal supports, a high temperature or extended time is required to completely remove the universal linker. For this reason it is inadvisable to use these supports with modifications that require mild, ultra mild or room temperature deprotection. Compatibility with RNA is therefore mixed, and we would not recommend use with TBDMS RNA chemistry.

Ref:

  1. A universal support for oligonucleotide synthesis, S. Scott, P. Hardy, R.C. Sheppard, and M.J. McLean, in Innovation and Perspectives in Solid-Phase Synthesis. Peptides, Proteins, and Nucleic Acids, Biological and Biomedical Applications (R. Epton, ed.), 115-124, 1994. Mayflower Worldwide, Ltd., Birmingham, UK.
  2. Hydroquinone-O,O'-diacetic acid as a more labile replacement for succinate acid linkers in solid-phase oligonucleotide synthesis, R.T. Pon and S.Y. Yu, Tetrahedron Lett., 38, 3327-3330, 1997.

Applicable Products

LK2300 Universal-Q SynBase™ CPG 500/110
LK2304 Universal SynBase™ CPG 1000/110
LK2410 Universal-Q SynBase™ CPG 1000/110 S
LK2411 Universal-Q SynBase™ CPG 1000/110 H
LK2458 Universal-Q Polystyrene

General

Rather than having predetermined bases or modifications on the solid support, the use of Universal Supports allows the addition of 3’- base or modification to the support. This is useful for many reasons. It allows preparation of plates (96, 384 etc) in an easy and efficient manner without the risk of the wrong solid support being added to the well. It also allows the incorporation of base modifications at the 3’-end of an oligo where no modified solid support is commercially available, e.g. 2’-F-dU.

Deblocking

An initial additional detritylation is recommended as this step is much slower on universal supports than on standard base supports. This prevents N-1 oligos from the 3’-end.

Coupling

No changes are required from the standard synthesis method recommended by the synthesiser manufacturer. This is therefore determined by the oligo type and any modification present.

Oxidation

LK2304 - No changes are required from the standard synthesis method recommended by the synthesiser manufacturer.
LK2300/LK2410/LK2411/LK2458 - The linker is stable to capping mixtures, but is slightly labile in oxidiser solution (8% cleavage overnight which is the equivalent of approximately 2000 synthesis cycles on an average program).

Cleavage & Deprotection

LK2304 - In order to completely remove the universal linker one of the following need to be used: (1) ammonium hydroxide solution, 17h at 80°C; (2) AMA, 5h at 80°C; or (3) AMA, overnight at 55°C.
LK2300/LK2410/LK2411 - Contrary to previously published mild methods, the best conditions we have found to completely remove the Q linker during deprotection are AMA at 70°C for 2.5h or AMA at 80°C for 2h. For this reason it is inadvisable to use this support with modifications that require mild, ultramild or room temperature deprotection. Compatibilty with RNA is therefore mixed. We would not recommend the use of the support with TBDMS chemistry.

Storage & Stability

Refrigerate at 2-8°C. Material is stable for several years.

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