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  6. N Mix Glyc-CPG (5'-DMT-[dA(Bz), dC(Ac), dG(dmf), T]-Glyc-CPG)

N Mix Glyc-CPG (5'-DMT-[dA(Bz), dC(Ac), dG(dmf), T]-Glyc-CPG)

N Mix Glyc-CPG (5'-DMT-[dA(Bz), dC(Ac), dG(dmf), T]-Glyc-CPG)

N Mix Glyc-CPG (5'-DMT-[dA(Bz), dC(Ac), dG(dmf), T]-Glyc-CPG), 1000 Å, Standard Loading, 1 g

CPG for incorporation of mixed unmodified dN at 3' end of an oligonucleotide.

Key features

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  • CPG mix has glycolate linkers for use with mild deprotection conditions
  • Available in smaller pack sizes suitable for research use
Option 1: Select a Pore Size
Option 2: Select a Functional Loading
Option 3: Select a Size
Item ID BG1-1400G-1
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Product information

A number of linker variants are used in our DNA CPG products, although a long-chain alkyl amino succinyl linker is the most common. The legacy Biosearch DNA CPGs typically use the AMP-succinyl combination, but sometimes can have a glycolate linker which cleaves under mild deprotection conditions. Similarly, the Q-linker (hydroquinone-0,0'-diacetic acid group) CPGs are advantageous for quick cleavage (2 minutes at room temperature) making them ideal for base sensitive oligonucleotides or dye labelled oligos. Pore sizes commonly available are 500 Å, 1000 Å, 1400 Å and 2000 Å, with application use similar to the Prime and SynBase™ products. These CPGs utilise a different raw glass to the Prime Synthesis products, but are generally offered in smaller pack sizes for research purposes.

Properties:

  • Appearance: white powder

Product usage:

  • Cleavage conditions: Use concentrated ammonia for 45 minutes at 25 °C or 1:1 ammonia:methylamine (AMA) for 15 minutes at 25 °C when using fast deprotecting amidites.
  • Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C.
    • This support should be stored at -20°C. If the product is stored at higher temperatures for prolonged periods of time the glycolate linkage may cleave resulting in lower yields. The C- Acetyl group may also come off as a result of prolonged exposure to higher temperatures. This product is temperature sensitive.

Storage and handling:

  • Shipping conditions: Cold
  • Storage conditions: -15 to -30 °C in sealed container

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