This phosphoramidite allows for efficient incorporation of a fluorescent deoxycytidine analogue into oligonucleotides.
Phosphoramidite for the efficient incorporation of a fluorescent deoxycytidine analogue into oligonucleotides.
Fluorescent nucleosides with etheno-bridged rings are useful in observing the transition between DNA structural types.
The intrinsic fluorescence of modified bases with etheno-bridged rings is useful in assessing the structure, dynamics and interactions of nucleic acids. Several of our modified nucleosides can be incorporated multiple times into DNA with retention of DNA structure and nucleobase recognition.
LGC, Biosearch Technologies offers several quality-controlled, functionally-validated fluorescent nucleosides available in multiple units and package sizes.
|Incorporate an etheno bridged dG moiety internally or at the 5’ end of an oligonucleotide.
|Efficiently incorporate a fluorescent deoxycytidine analogue into oligonucleotides.
|5,N4-Etheno-dC CE-Phosphoramidite (i.e., Pyrrolo-dC CEP)
|Efficiently incorporate a fluorescent deoxycytidine analog into oligonucleotides.
For more information, download the Product Information Sheet.
3,N4-Etheno-dC CEP and 5,N4-Etheno-dC CEP are fluorescent pyrimidine nucleoside analogues that are useful for investigating nucleic acid interactions. Due to their similarity in structure to dC, the modified nucleosides can be incorporated into DNA without perturbing DNA structure and nucleobase recognition.4
- Shanmugam, G.; Kozekov, I. D.; Guengerich, F. P.; Rizzo, C. J.; Stone, M. P. Chem. Res. Toxicol. 2008, 21, 1795-1805.
- Shanmugam, G.; Goodeneough, A.K.; Kozekov, I. D.; Guengerich, F. P.; Rizzo, C. J.; Stone, M. P. Chem. Res. Toxicol., 2007, 20(11), 1601-1611.
- Stone, M. P.; Huang, H.; Brown, K. L.; Shanmigam, G. Chemistry & Biodiversity, 2011, 8, 1571-1615.
- Ming, X.; Seela, F. Chem. Eur. J. 2012, 18, 9590-9600 and references therein.