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Propyne dC (DBF) Amidite

Propyne dC (DBF) Amidite

Phosphoramidite used to incorporate a C-5 propyne modified dC either internally or at the 5' end of an oligonucleotide.
  • Enhances base pairing.
  • Confers duplex stability and melting temperatures are raised by 2.8° per substitution.
  • Confers nuclease resistance and binding affinity of antisense oligos.
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Product information

Substitution of C-5 propynyl-dC (pdC) for dC and C-5 propynyl-dU (pdU) for dT are effective strategies to enhance base pairing. Duplex stability and melting temperatures are raised by 2.8° or 1.7° per substitution of pdC or pdU respectively. Such Tm modification is used in Turbo Taqman probes for example. C5-propyne pyrimidine modifications are sometimes added to increase nuclease resistance and binding affinity of antisense oligos.

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    Image of cleaved and deprotected structure:

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