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dU CPG Column

dU CPG Column

CPG column used to incorporate a deoxyuridine base into the 3' end of an oligonucleotide.
  • Used to induce mutagenic effects.
  • The enzyme uracil-N-glycosylase (UNG) can specifically remove uracil to create abasic sites at the deoxyuridine positions. This property is used to generate site-specific strand breaks in the oligonucleotide.
  • CPG has long-chain alkylamino succinyl linker.
  • 1000 Å CPG suitable for highly modified oligonucleotides (> 20mers).
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Product information

Incorporation of a deoxyuridine base within a DNA sequence can be used to induce mutagenic effects. The enzyme uracil-N-glycosylase (UNG) can specifically remove uracil to create abasic sites at the deoxyuridine positions. This property is used to generate site-specific strand breaks in the oligonucleotide. We provide both dU phosphoramidite and CPGs.

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