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5'-DMT-dU Amidite (5'-DMT-deoxyUridine)

5'-DMT-dU Amidite (5'-DMT-deoxyUridine)

Phosphoramidite used to incorporate a deoxyuridine base into an oligonucleotide.
  • Used to induce mutagenic effects.
  • The enzyme uracil-N-glycosylase (UNG) can specifically remove uracil to create abasic sites at the deoxyuridine positions. This property is used to generate site-specific strand breaks in the oligonucleotide.
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Product information

Incorporation of a deoxyuridine base within a DNA sequence can be used to induce mutagenic effects. The enzyme uracil-N-glycosylase (UNG) can specifically remove uracil to create abasic sites at the deoxyuridine positions. This property is used to generate site-specific strand breaks in the oligonucleotide. We provide both dU phosphoramidite and CPGs.

Properties:

  • Formula: C39H47N4O8P
  • Molecular Weight: 730.31
  • Appearance: white solid

Product usage:

  • Dilution: 100 µmol/1 mL
  • Deprotection conditions: The deprotecting conditions for this amidite will depend on the types of amidites used for the synthesis of the oligo. If using fast deprotecting amidites deprotect in concentrated NH4OH for 1 hour at 60 °C. If using standard amidites deprotect in concentrated NH4OH for 5 hours at 60 °C.
  • Image of cleaved and deprotected structure:
  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 290.17

Storage and handling:

  • Shipping conditions: Cold
  • Storage conditions: -15 to -30 °C

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