Phosphoramidite for incorporation of Biotin with a dT nucleobases, internally or at the 5' end of an oligonucleotide.
Colourimetric Detection and Capture
For colorimetric detection and capture, hapten labeled probes are commonly generated with biotin and 2,4-dinitrophenyl (DNP). The probes get hybridized to an immobilised target and treated with substrate (such as avidin) to produce a distinctive colour.
LGC, Biosearch Technologies offers 3’, internal and 5’ biotinylation reagents, as well as biotin derivatives and DNP phosphoramidites, with various spacers for incorporation into oligos.
We offer a diverse array of biotinylation reagents for labelling nucleic acids. Choosing the right one largely depends on the position within the oligonucleotide you are labelling (3’-terminus, 5’-terminus, internal) as well as the spacer you need (carbon, triethylene glycol, etc).
|Biotin C6 T Amidite (TBB)
|Incorporates a biotin label with dT nucleobase and C6 spacer, either internally or at the 5' end of an oligonucleotide.|
|Adds multiple biotins to either the 3’ or 5’ end of an oligo.|
|Incorporates a biotin label with a triethylene glycol (TEG) spacer internally or at the 5' end of an oligonucleotide. The spacer arm separates the biotin function from the rest of the oligo.|
|Adds biotin to the 5’-end of an oligo. The DMT group prevents branching during coupling, and it can be used as a purification handle.|
|Incorporates desthiobiotin with TEG linker to the 5’- end of an oligo. Enables affinity capture using streptavidin.|
In addition to the phosphoramidites, we offer bulk CPG and CPG synthesis columns for directly incorporating biotin or desthiobiotin onto the 3’-end of your oligo.
- 3'-Biotin-TEG CPG
- Desthiobiotin-TEG CPG
Incorporating a dinitrophenyl-modification either internally or at the terminus of an oligonucleotide can enable detection using anti-DNP antibodies.
- Useful in colourimetric analytical detection methods.
- Tetraethylene glycol spacer affords hydrophilicity.
- Branched structure allows multiple additions at either 3' or 5' terminus.