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Colourimetric Detection and Capture

For colorimetric detection and capture, hapten labeled probes are commonly generated with biotin and 2,4-dinitrophenyl (DNP). The probes get hybridized to an immobilised target and treated with substrate (such as avidin) to produce a distinctive colour.

LGC, Biosearch Technologies offers 3’, internal and 5’ biotinylation reagents, as well as biotin derivatives and DNP phosphoramidites, with various spacers for incorporation into oligos.

Biotinylation Reagents

We offer a diverse array of  biotinylation reagents for labelling nucleic acids. Choosing the right one largely depends on the position within the oligonucleotide you are labelling (3’-terminus, 5’-terminus, internal) as well as the spacer you need (carbon, triethylene glycol, etc).

Reagent Description
Biotin C6 T Amidite (TBB)
Incorporates a biotin label with dT nucleobase and C6 spacer, either internally or at the 5' end of an oligonucleotide.
Biotin-CE Phosphoramidite
Adds multiple biotins to either the 3’ or 5’ end of an oligo.
Biotin-TEG-CE Phosphoramidite
Incorporates a biotin label with a triethylene glycol (TEG) spacer internally or at the 5' end of an oligonucleotide. The spacer arm separates the biotin function from the rest of the oligo.
5’-Biotin-CE Phosphoramidite
Adds biotin to the 5’-end of an oligo. The DMT group prevents branching during coupling, and it can be used as a purification handle.
Desthiobiotin-TEG CE-Phosphoramidite
Incorporates desthiobiotin with TEG linker to the 5’- end of an oligo. Enables affinity capture using streptavidin.

In addition to the phosphoramidites, we offer bulk CPG and CPG synthesis columns for directly incorporating biotin or desthiobiotin onto the 3’-end of your oligo. 

  • 3'-Biotin-TEG CPG
  • Desthiobiotin-TEG CPG
  • DMT-C3(Biotin)-Suc-CPG

DNP-TEG CE-Phosphoramidite

Incorporating a dinitrophenyl-modification either internally or at the terminus of an oligonucleotide can enable detection using anti-DNP antibodies.

  • Useful in colourimetric analytical detection methods.
  • Tetraethylene glycol spacer affords hydrophilicity.
  • Branched structure allows multiple additions at either 3' or 5' terminus.