Solid Chemical Phosphorylating Reagent (solidCPR)
CAS No.:202284-84-2
Phosphoramidite for 5' phosphorylation of an oligonucleotide.
Phosphoramidite for 5' phosphorylation of an oligonucleotide.
Phosphoramidite for 5' phosphorylation of an oligonucleotide.
Phosphoramidite for 5' or 3' phosphorylation of an oligonucleotide.
Oligonucleotides containing a 5'-phosphate group have various applications, and are often used as a means of ligating one oligo to another, e.g. as linkers and adapters, in cloning, gene construction, and ligation in general.
3'-Phosphorylations are used to block enzyme activity. For example, this is an efficient and commonly used PCR blocking technique.
Phosphorylation of the 5'-terminus on oligonucleotides is routinely achieved, with higher yields than using kinase, using a Chemical Phosphorylation Reagent (also known as CPR or “Phosphate-ON”)1.
Chemical phosphorylation reagents for 5'-terminus modification
Product | Description and Advantages |
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Chemical Phosphorylating Reagent (CPR) NACE1-008 |
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5'-Phosphorylating Amidite II NACE1-006 |
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Chemical Phosphorylating Reagent (CPR II)2 NACE1-007 |
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Solid Chemical Phosphorylating Reagent (solid CPR)3 NACE1-011 |
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3'-Phosphate CPGs allow direct preparation of oligonucleotides with a 3'-phosphate group.
Chemical phosphorylation reagents for 3'-terminus modification
Product | Pore Size Availability |
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3'-Phosphate CPG NACE1-002 |
500 Å, 1000 Å, 3000 Å |
3'-Phosphate CPG Column NACE1-003 |
1000 Å, 3000 Å (available in different column formats) |
DMT-Phosphate-Suc-CPG NACE1-009 |
500 Å, 1000 Å |
DMT-Phosphate-Suc-CPG Column Suc-CPG) NACE1-010 |
1000 Å, 1400 Å (available in different column formats) |
CPG Pore Size | Application |
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500 Å | Suitable for high yield applications such as therapeutic oligos (≤ 30mers) |
1000 Å | Suitable for highly modified oligonucleotides (> 20mers) |
3000 Å | Performs well for very long sequences (> 120mers) |
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