Phosphoramidite for 5' or 3' phosphorylation of an oligonucleotide.
Oligonucleotides containing a 5'-phosphate group have various applications, and are often used as a means of ligating one oligo to another, e.g. as linkers and adapters, in cloning, gene construction, and ligation in general.
3'-Phosphorylations are used to block enzyme activity. For example, this is an efficient and commonly used PCR blocking technique.
Phosphorylation of the 5'-terminus on oligonucleotides is routinely achieved, with higher yields than using kinase, using a Chemical Phosphorylation Reagent (also known as CPR or “Phosphate-ON”)1.
Chemical phosphorylation reagents for 5'-terminus modification
|Product||Description and Advantages|
|Chemical Phosphorylating Reagent (CPR)
|5'-Phosphorylating Amidite II
|Chemical Phosphorylating Reagent (CPR II)2
|Solid Chemical Phosphorylating Reagent (solid CPR)3
3' Phosphate Modification of an Oligonucleotide
3'-Phosphate CPGs allow direct preparation of oligonucleotides with a 3'-phosphate group.
Chemical phosphorylation reagents for 3'-terminus modification
|Product||Pore Size Availability|
|500 Å, 1000 Å, 3000 Å|
|3'-Phosphate CPG Column
|1000 Å, 3000 Å (available in different column formats)|
|500 Å, 1000 Å|
|DMT-Phosphate-Suc-CPG Column Suc-CPG)
|1000 Å, 1400 Å (available in different column formats)|
Our 3’-Phosphate CPGs are available in different pore sizes:
|CPG Pore Size||Application|
|500 Å||Suitable for high yield applications such as therapeutic oligos (≤ 30mers)|
|1000 Å||Suitable for highly modified oligonucleotides (> 20mers)|
|3000 Å||Performs well for very long sequences (> 120mers)|
- A chemical 5'-phosphorylation of oligodeoxyribonucleotides that can be monitored by trityl cation release, T. Horn and M. Urdea, Tetrahedron Lett., 27, 4705-4708, 1986.
- A new approach for chemical phosphorylation of oligonucleotides at the 5'-terminus, A. Guzaev, H. Salo, A. Azhayev and H. Lonnberg, Tetrahedron, 51, 9375-9384, 1995.
- Chemical phosphorylation of oligonucleotides and reactants therefor, A. Guzaev, A. Azhayev and H. Lonnberg, US Patent No. 5959090, 1999.