Luminol is one of the most widely used chemiluminescent substrates, and luminol-based indirect bioassays are linear and efficient. 3-Aminophthalimide derivatives are considered to be “pro-chemiluminescent” substrates since treatment with hydrazine forms a primary amine and free luminol.⁽¹⁾ These derivatives are also highly fluorescent and can be used for dual fluorescence and chemiluminescence studies.
Our Luminol synthon NHS ester is designed for post-synthetic modification of an oligonucleotide which can then be used as a fluorescent tagged oligo, or treated with hydrazine for chemiluminescence studies.

Ref:

1. Mavri-Damelin, D.; Wilden, J.; Mani, A.-R.; Selden, C.; Hodgson, H.J.F.; Damelin, L.H. Bioconjugate Chem. 2009, 20, 266-273.