Skip to content Skip to navigation menu

Exo-Minus Klenow DNA Poly (D355A, E357A)

Exo-Minus Klenow DNA Poly (D355A, E357A)

A Klenow DNA polymerase lacing all 5' to 3' and 3' to 5' exonuclease activity.
  • A Klenow DNA polymerase lacking all 5' to 3' and 3' to 5' exonuclease activity
  • Synthesise DNA from ssDNA templates without degrading any single-stranded DNA
  • Suitable for a variety of applications including random-primer labelling of DNA,1second-strand cDNA synthesis,2sStrand-displacement amplification3

Size: 1000 units

Item ID KL11101K
TBD
Add to basket to request a quote

Product information

Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´ to 3´ and 3´ to 5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I also has two mutations (D355A and E357A).

References

  1. Feinberg, A.P. and Vogelstein, B. (1984) Anal. Biochem. 137, 266.
  2. Sanbrook, J. et al. (1989) In: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York. v.2, p. 8.14.
  3. Walter, N.G. (1995) J. Mol. Biol. 254, 856.

Unit Definition: One unit of Exo-Minus Klenow DNA Polymerase (D355A, E357A) converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37 °C under standard assay conditions.

Quality Control: Exo-Minus Klenow DNA Polymerase (D355A, E357A) is free of detectable endo- and exonuclease and RNase activities.

Manuals and user guides

Product information sheets

Access support

Need some support with placing an order, setting up an account, or finding the right protocol?

Contact us