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TNP-AECM-FICOLL

TNP-AECM-FICOLL

Trinitrophenyl hapten is conjugated to AminoEthylCarboxyMethyl-FICOLL (AECM-FICOLL).
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Product information

LGC Biosearch Technologies offers hapten conjugated proteins; unlabeled proteins (OVAL; BSA ; CGG and KLH) and fluorescent protein conjugates for immunochemistry. We also offer the haptens that can be used as labeling reagents for custom conjugates. TNP (2,4,6-Trinitrophenyl) hapten is conjugated to AminoEthylCarboxyMethyl-FICOLL (AECM-FICOLL), a high molecular weight polysaccharide. Hapten-FICOLL conjugates are useful as T-independent antigens for studying B lymphocyte activation.

Properties:

  • Chemical Name: 2,4,6-Trinitrophenyl-AminoEthylCarboxyMethyl-FICOLL
  • Appearance: Orange powder
  • Absorption Maximum (Lambda Max): 348
  • Extinction Coefficient at Lambda max: 15400

Product usage:

    This product can be dissolved in water or 0.15M phosphate buffered saline (PBS) pH 7.2 at a concentration of 1 mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant.Store solution in aliquots at -20°C. Protect from light.Conjugation of TNP is given as a molar ratio of TNP to Ficoll assuming the molecular weight of Ficoll is 400,000. It is determined at 348 nm using 1.54 x 10E+04 as the extinction coefficient.

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: Room Temperature

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