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PC-KLH (Keyhole Limpet Hemocyanin)

PC-KLH (Keyhole Limpet Hemocyanin)

PC-KLH (Keyhole Limpet Hemocyanin), 5 mg, ABI (5 mL / 20 mm Septum)

Phosphorylcholine hapten is conjugated to KLH protein by use of p-diazonium phenylphosphorylcholine.
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Item ID PC-1013-5
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Product information

Our line of conjugates include well studied small molecule haptens eg, DNP, TNP, NP, NIP, and PC (Phosphoryl Choline). Various levels of conjugation density are available depending on the antibody response required by the researcher. Such reagents are complete antigens useful for inducing antibody response in animal models. Conjugation ratio is the molar ratio of hapten to protein. The conjugation ratio (or "loading") for any given immunochemical will vary with each production batch, and we do not offer customised ratios. Where standard ratio ranges are not offered, customer service can inform you about available ratios at the point of purchase.

Properties:

  • Chemical Name: Phosphorylcholine Keyhole Limpet Hemocyanin
  • Formula: C11H17N3O4P-KLH
  • Molecular Weight: 286.25
  • Appearance: orange powder
  • Absorption Maximum (Lambda Max): 475
  • Extinction Coefficient at Lambda max: 12600

Product usage:

    Conjugation is determined at pH 12 by measuring PC absorbance at 475nm, using the extinction coefficient of PC= 12.6 x 10E+03. The conjugation ratio is given as a molar ratio of PC to KLH assuming the molecular weight of KLH is 100,000.Store solution in aliquots at +5°C. KLH may come out of solution if frozen. Protect from light. This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant.When using PBS please refer to the formulation below.PBS Formulation:8 g NaCl0.2 g KCl0.2 g potassium di-hydrogen phosphate (KH2PO4)1.15 g di-sodium hydrogen phosphate (Na2HPO4)Add to 1 L of water

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: +2 to +8 °C

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