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NIP-OSu

NIP-OSu

NIP-OSu, 100 mg, ABI (5 mL / 20 mm Septum)

4-Hydroxy-3-iodo-5-nitrophenyl active ester is useful for conjugation to proteins, in order to form immunogenic agents used to raise antibodies.
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Item ID N-1080-100
TBD
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Product information

Hapten reagents (such as succinimide esters) allow the customer to synthesize their own custom conjugates.

Properties:

  • Chemical Name: 4-Hydroxy-3-iodo-5-nitrophenylacetic acid Succinimide Ester
  • Formula: C12H9IN2O7
  • Molecular Weight: 420.12
  • Appearance: yellow powder
  • Absorption Maximum (Lambda Max): 430
  • Extinction Coefficient at Lambda max: 5000

Product usage:

    For conjugation to protein or DNA, dissolve in DMSO.NIP OSu Conjugation to a protein:Add NIP OSu to protein pre-dissolved in HCO3- buffer (pH ca 8.0) (Protein Concentration: Approx. 10 mg/mL). Stir 1 day at room temperature. Spin down insoluble material. Decant soluble fraction and dialyze until no color escapes (2-3 days). Lyophilize. Determine substitution ratio @430 nm, pH 8.5Take a spectral scan of the solution using a spectrophotometer.Absorbance should be less than 1.0.Use the absorbance value at 430 nm to determine the loading of NIP per unit of OSu.

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: -15 to -30 °C

SDS

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