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NP-OSu

NP-OSu

NP-OSu, 100 mg, ABI (5 mL / 20 mm Septum)

4-Hydroxy-3-nitrophenyl hapten active ester is useful for conjugating to proteins in order to form immunogenic agents used to raise antibodies.
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Item ID N-1010-100
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Product information

Hapten reagents (such as succinimide esters) allow the customer to synthesize their own custom conjugates.

Properties:

  • Chemical Name: 4-Hydroxy-3-nitrophenylacetic acid Succinimide Ester
  • Formula: C12H10N2O7
  • Molecular Weight: 294.22
  • Appearance: pale yellow powder
  • Absorption Maximum (Lambda Max): 430
  • Extinction Coefficient at Lambda max: 4230

Product usage:

    For conjugation to protein or DNA, dissolve in DMSO.NP OSu Conjugation to a protein:Add NP OSu to protein pre-dissolved in HCO3- buffer (pH ca 8.0) (Protein Concentration: Approx. 10 mg/mL). Stir 1 day at room temperature. Spin down insoluble material. Decant soluble fraction and dialyze until no color escapes (2-3 days). Lyophilize. Determine substitution ratio @430 nm, pH 8.5Take a spectral scan of the solution using a spectrophotometer.Absorbance should be less than 1.0.Use the absorbance value at 430 nm to determine the loading of NP per unit of protein.

Storage and handling:

  • Shipping conditions: Ambient
  • Storage conditions: -15 to -30 °C

SDS

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