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BigEasy-TSA Electrocompetent Cells

Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

BigEasy-TSA Electrocompetent Cells

Transform and propagate pJAZZ Vector clones and libraries with this unique E. coli competent cell strain.
  • Cells required for transformation and propagation of pJAZZ® Vectors.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimised for high yields of DNA.
Select a Size
TBD
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Product information

BigEasy™-TSA™ Electrocompetent Cells (>2 × 1010 cfu/µg pKanR DNA) are for use with pJAZZ®-OC or OK Linear Vectors in the BigEasy v2.0 Linear Cloning System. The TSA Cells have the telN gene required for high efficiency transformation, and the antA gene required for copy number control with the pJAZZ vector, integrated into the host genome. These cells also give high yield and high quality plasmid DNA due to the endA1 and recA1 mutations. They contain the mcr and mrr mutations, allowing methylated genomic DNA that has been isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements.

Table 1. E. cloni Competent Cells for BAC, Large or Difficult Fragment Cloning


E. cloniCell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

BigEasy-TSA Electrocompetent

>2 × 1010

YES

No

YES
IPTG induction required

BigEasy-TSA: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA AmpR sopAB telN antA

BigEasy-TSA: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA AmpR sopAB telN antA

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