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BAC-Optimised Electrocompetent Cells

Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube) or FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

BAC-Optimised Electrocompetent Cells

  • Cells that enable the most challenging library construction and screening.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimised for high yields of DNA.
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Product information

We offer two bacterial strains designed for BAC library construction or transformation of large constructs:

BAC-Optimised Replicator™ v2.0 Electrocompetent Cells (> 1 × 1010 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are components of Biosearch Technologies Cloning Kits

E. cloni 10G BAC-Optimised Electrocompetent Cells (> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed specifically for large DNA cloning. Manufactured using a novel procedure that maximises transformation efficiencies with very large inserts, 10G BAC-Optimised Cells are an excellent choice for BAC library construction and large construct transformation.

Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning


E. cloni Cell Lines

Transformation Efficiency
(cfu/µg pUC or pKanR DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

10G BAC-Optimised Electrocompetent

>1 × 1010

YES

YES

YES
without IPTG induction

BAC-Optimised Replicator v2.0 Electrocompetent

>1 × 1010

YES

YES

YES
IPTG induction required

Replicator v2.0: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galUgalrpsnupG (attaraC-PBAD-trfA250 bla attR) λ-. Note that Replicator v2.0 cells are AmpR (bla gene) (StrR)

E. cloni 10G BAC-Optimized: F - mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)

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