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E. cloni 5-alpha Chemically Competent Cells

Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

E. cloni 5-alpha Chemically Competent Cells

Clone and subclone with high efficiency, chemically competent DH5-alpha cells.
  • Direct replacements for standard cloning strains
  • Optimised genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.
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Product information

E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.

E. cloni 5-alpha Chemically Competent Cells >1 × 10 8 cfu/µg DNA
Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.

Table 1. E. cloni Competent Cells for Cloning


E. cloni Cell Lines

Transformation Efficiency
(cfu/µg pUC DNA)

Cloning Methylated DNA

BAC, Cosmid Cloning

Blue/White Screening

5-alpha Chemically Competent

>1 × 10 8 cfu/µg DNA

NO

NO

YES
No IPTG induction required

Genotype

E. cloni 5-alpha: fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17


Efficiencies

E. cloni 5-alpha Chemically Competent Cells >1 × 108 cfu/µg DNA

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