Clone and subclone with high efficiency, chemically competent DH5-alpha cells.
Clone unstable and toxic sequences in standard plasmids, with high efficiency.
Eliminate endotoxin at the source with this unique engineered E. coli strain for low-endotoxin protein expression.
Clone large inserts in standard plasmids, avoid T1 and T5 phage contamination, and control copy number of CopyControl vectors.
Create large phage display libraries with high efficiency cells recommended for peptide display.
Induce CopyControl Fosmid clones to higher-copy number.
Stabilise toxic sequences behind T7 or lac-based promoters with this strain containing a LacI repressor, providing tight control over leaky expression.
Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.
Clone large inserts in standard plasmids, and control copy number of CopyControl vectors. EPI300 Electrocompetent cells are recommended for Gibson Assembly® reactions.
Clone and sub-clone with high-efficiency, recA, endA competent cells.
Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.
Get better colony counts for routine protein expression with any E. coli T7 expression vector, with this high-efficiency BL21(DE3) strain.
Induce high-level protein expression from T7 promoters with tight control over leaky expression, using this strain containing a LacI repressor.
Optimised formulation for ensuring stability and high-efficiency transformation of protein expression clones.
Induce plasmid copy number in CopyCutter EPI400 strain, for high plasmid yields.
Transform and propagate pJAZZ Vector clones and libraries with this unique E. coli competent cell strain.
Construct larger, more complex phage display libraries with highest efficiency cells. This amber suppressor strain can be used for phage display and protein expression.
High efficiency F-control strain for phage display applications.
Express difficult or toxic proteins using standard E. coli T7 expression vectors using these unique strains. Also available in chemically competent format.
Construct larger, more complex phage display libraries with highest efficiency cells. SS320 is a non-amber suppressor strain used for phage display.
Competent cells for transformation
LGC, Biosearch Technologies offers the best performance, value, and convenience in competent cells for cloning and expression. Our chemically competent cells offer an unmatched value for routine cloning, and our electrocompetent cells provide the highest possible efficiency for difficult cloning and library construction.
See our Custom Competent Cell Services page to learn how you can receive the high-quality cells in the exact format you need, when you need them. Custom dispensing is available in tubes or 96-well plates, for your strain or ours.
ISO 13485 Certified with 10+ years of manufacturing excellence
Our competent cells must pass strict QC specifications for transformation efficiency and antibiotic resistance.
Every lot of cells is tested to ensure the cells meet our guaranteed transformation efficiencies. Cells are also tested for resistance to a panel of antibiotics by plating mock-transformed cells on antibiotic plates. Common antibiotic panel includes: kanamycin, carbenicillin (ampicillin), streptomycin, tetracycline, gentamycin and chloramphenicol.
Chemically competent cells vs. Electrocompetent cells
|Chemically competent cells||Electrocompetent cells|