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MaxPlax Lambda Packaging Extracts

Contents: Pre-dispensed MaxPlax Extracts, Control Lambda DNA, Control Host Cells (E. coli).

MaxPlax Lambda Packaging Extracts

Package fosmid, cosmid or lambda DNA to generate genomic libraries.
  • Maximised packaging efficiency of lambda, cosmid, or fosmid DNA for the construction of large insert genomic libraries.
  • Construct genomic libraries from methylated or unmethylated DNA with equal efficiency due to the lack of all known restriction activities in the extracts.
  • Packaging efficiencies of up to 3 × 109 pfu/µg DNA
  • Simplified protocol does not require premixing of different extract components before use.
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Product information

MaxPlax™ Lambda Packaging Extracts offer maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E. coli strains, BHB2690 and BHB2688, as described by Hohn.1 MaxPlax Extracts use a restriction-free E. coli K-12 strain, NM759, in place of strain BHB2690.2 The sonicated extracts of NM759 are combined with extracts of BHB2688 to produce MaxPlax Extracts.

Each lot of MaxPlax Extracts is tested and guaranteed to maintain a packaging efficiency of greater than 1 × 109 pfu/µg of Control Ligated Lambda DNA for 1 year from the date of purchase if stored properly. A Certificate of Analysis stating actual packaging efficiency (pfu/µg DNA) is provided with each lot of MaxPlax Extracts.

Table 1. Packaging of methylated and unmethylated DNA using MaxPlax Lambda Packaging Extracts.
Cosmid Insert Pfu/µg DNA
Unmethylated DNA 3.5 x 107
Methylated DNA 3.9 x 107

References

  1. Hohn, E.G. (1979) Methods Enzymol. 68, 299.
  2. Gunther, E.G. et al. (1993) Nucleic Acids Res. 21, 3903.
  3. Fiandt, M. (2000) Epicentre Forum 7(3), 14.

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