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End-It DNA End-Repair Kit

Contents: End-Repair Enzyme Mix, End-Repair 10X Buffer, dNTP Solution, ATP.

End-It DNA End-Repair Kit

Prepare blunt, phosphorylated DNA ends from PCR product or sheared DNA with this repair kit.
  • Convert DNA with damaged or incompatible 5´- and/or 3´-protruding ends into ready-to-ligate, 5´-phosphorylated, blunt-ended DNA.
  • Complete conversion of DNA into blunt-ended, phosphorylated DNA
  • Repair sheared, nebulised or restriction enzyme-digested genomic DNA
  • Prepare templates for NGS (next-generation sequencing) or shotgun library preparation
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Product information

The End-It™ DNA End-Repair Kit converts DNA containing damaged or incompatible 5´- and/or 3´-protruding ends to 5´-phosphorylated, blunt-ended DNA. The end-repaired DNA can be rapidly ligated into a cloning vector or to next-gen sequencing adaptors, using the Fast-Link™ DNA Ligation Kit.

The End-It DNA End-Repair Kit contains reagents for 20 or 50 end-repair reactions (repair of up to 100 µg or 250 µg of genomic DNA, respectively).

Figure 1. DNA fragments containing any type of ends are rapidly and efficiently converted to 5´-phosphorylated, blunt-ended DNA using the End-It DNA End-Repair Kit. The end-repaired DNA is ready for blunt-end ligation using, for example Epicentre's Fast-Link DNA Ligation Kit.

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